APOC1 ELISA Kit

Produktnummer ABIN6730064

Dieses Human APOC1 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human APOC1 zu quantifizieren.

Kurzübersicht für APOC1 ELISA Kit (ABIN6730064)

Target: APOC1 (Apolipoprotein C-I (APOC1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum

Produktdetails APOC1 ELISA Kit

Verwendungszweck: Human ApoC1 ELISA Kit. The SpeedELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of target protein in biological sample such as serum, plasma, and cell culture supernatants.

Marke: SpeedELISA

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human ApoC1. Other species not determined yet.

Produktmerkmale:

• Compressed workflow with only one wash step and three incubation steps
• 3 hours total processing time
• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Streptavidin-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Capture Antibody
• HRP Conjugated Detection Antibody
• TMB One-Step Substrate

Benötigtes Material:

1. Microplate reader capable of measuring absorbance at 450 nm.
2. Precision pipettes to deliver 2 µl to 1 ml volumes.
3. Adjustable 1-25 ml pipettes for reagent preparation.
4. 100 ml and 1 liter graduated cylinders.
5. Absorbent paper.
6. Distilled or deionized water.
7. Log-log graph paper or computer and software for ELISA data analysis.
8. Tubes to prepare standard or sample dilutions.

Anwendungsinformationen

Testdauer: 3 h

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 70 µl of the Antibody Cocktail to each well.
3. Incubate 10 minutes at RT.
4. Add 50 µl of sample or standard to each well.
5. Incubate 2 h at RT.
6. Wash Wells
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment.Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C. For prepared reagent storage, see table below.

Haltbarkeit: 6 months

Details zu APOC1

Target: APOC1 (Apolipoprotein C-I (APOC1))

Andere Bezeichnung: ApoC1

Pathways: Apoptose